Baculoviruses offer many advantages over other expression vector systems, including safety, ease of scale-up, high levels of recombinant gene expression, and accuracy. In the present study, Lh of the orange-spotted grouper was synthesized using the Bac-to-Bac baculovirus expression system.
It has been accepted that FSH is involved in the control of puberty and gametogenesis and LH regulates the maturation of the ovary and spermatogenesis in mammals.
Gonadotropins stimulate androgen synthesis from cholesterol by binding to the receptor on the oocyte in the ovary, and then androgen is converted into estrogen, catalyzed by aromatase, and finally facilitates the synthesis of hepatic vitellogenesis. Aromatase P450 is an essential component of the important enzyme complex which catalyzes the conversion of androgen into estrogen. As in some other teleost fishes, the orange-spotted grouper has two distinct cDNA forms of aromatase P450-cyp19a1a and cyp19a1b-which mainly express in the ovary and the brain, respectively. Gonadotropins may activate the hormone-sensitive adenylate cyclase (AC) via Gs protein leading to formation of cAMP, activation of PKA and CRE, and modulation of gene expression associated with enhanced steroidogenesis.