Weights of pituitary, brain, ventral prostate, dorsolateral prostate, seminal vesicles (wet and dry), testes, epididymides, levator ani plus bul-bocavernosus muscles, bulbourethral glands, adrenals, liver, and kidneys were also taken. In females, brain and pituitary weights were measured and reproductive tissues were examined for malformations. In the pilot study, the offspring data were analyzed using individual and litter mean values.
Main Study Treatment Assignment and Dosing
In an attempt to ensure that all dams were pregnant, the 8 (of 48) dams showing the smallest weight gain between GDs 3 and 13 were eliminated from the study. On GD 13, the rats were randomly assigned to treatment groups in a manner that provided each group with equal means and standard errors for body weight. Animals were assigned to treatment groups for a total of eight experimental blocks (each block contained one dam from each treatment group). Thus the final randomized block design consisted of five treatment groups with eight dams per group. Animals were distributed on cage racks according to experimental block (i.e., animals in the same block were housed in closest proximity).