Offspring Effects During Puberty and Adult Life
In the female offspring, pubertal landmarks, as well as age and body weight at vaginal opening, were not affected by prenatal PZ exposure. In the male offspring, 1 of 47 controls showed a persistent preputial thread (incomplete PPS) until PCD 82 (PND 60), but the prepuce was separated at the time of necropsy. Two of 46 (4.35%) 62.5 mg/ kg-BW per day-treated animals (from the same litter) showed incomplete PPS until PCD 82. A large preputial thread on one of the animals persisted to necropsy (PCD 169). In the 125 mg/kg-treated group, all males in one litter (four pups) and one male in another litter showed incomplete PPS until PCD 82, which persisted until necropsy (PCDs 147-213; Fig. 5).
In all members of the 250 mg/kgtreated group, phalli were so malformed that preputial separation could not be fully assessed until necropsy. In pups with normal phallic morphology, there was no effect of treatment on age at separation at PZ doses of 125 mg/kgBW per day or lower.
Necropsy of Mature Male Offspring
All surviving males born to dams treated with 250 mg/ kg-BW per day retained nipples as adults. Permanently retained nipples were observed in males from all PZ-treated dosage groups, but the effect was statistically significant only at the highest dose.