Males displayed areolas/ nipples on PCD 35 (PND 13) at frequencies of 40%, 71%, and 100% in the 62.5, 125, and 250 mg/kg-BW per day treatment groups, respectively. When necropsied as adults, the male offspring displayed reduced weight of the levator ani plus bulbocavernosus muscles in a dose-related fashion. At the time of necropsy, nipple retention in male offspring was observed at frequencies of 10%, 14%, and 100% in the 62.5, 125, and 250 mg/kg-BW per day groups, respectively.
One-third of males in the 250 mg/kg-BW per day treatment group showed incomplete PPS, and the same percentage (including one animal with normal PPS) displayed a group of phallus abnormalities that included cleft phallus and hypospadias/epispadias.
Main Study: Maternal Effects of PZ
Although a dose-dependent reduction in weight gain was detected in dams between GD 14 and GD 22, the effects of treatment on weight gain were not statistically significant. Time to parturition increased significantly with dose, with dams in the 250 mg/kg-BW per day group initiating delivery more than 24 h after dams in other treatment groups.
FIG. 2. PZ competitive inhibition of [3H]R1881 binding to the rat androgen receptor in cytosolic preparations from ventral prostate tissue. Results are from three sets of assays run in duplicate. Analysis was conducted using Sigmaplot Ligand software (available on the web at http:// www.statsol.ie/sigmaplot/ligand.htm). Error bars = SEM.