Wet and dry weights were recorded for the reproductive tract (oviducts, uterus, cervix, and vagina), ovaries, adrenals, liver, and kidney. As the examination of control and high-dose animals revealed kidney weight and body weight differences among treatment groups, all remaining control, low-dose, and mid-dose females were examined (using the remainder of the randomized blocks) for body weight, liver weight, and kidney weight. Female necropsies were completed on PCD 255.
Data were analyzed using PROC GLM from SAS version 6.08 on the U.S. Environmental Protection Agency IBM mainframe computer. Statistically significant effects (F values) were further examined using the LSMEANS (two-tailed f-test) procedure to compare groups. If PZ demasculinized the male offspring, we expected to see a dose-related decrease in androgen-dependent tissue measures and increases in retained nipples and reproductive tract malformations. If the increase in anogenital distance at PCD 25 truly represented masculinization of the female offspring, we expected to see increases in reproductive tract malformations (vaginal agenesis, retained male sex accessory tissues) and reductions in the number of nipples.