GM-CSF Immunostaining Is Increased in LE During Early Pregnancy
The intensity of the red staining (Fig. 4) in the LE and S was evaluated by image analysis, first by comparing between days of the estrous cycle or pregnancy. In the LE (Fig. 5A), no significant effect of day was observed during the cycle (regression analysis, P > 0.5). On the other hand, GM-CSF was significantly stimulated during pregnancy in the LE from d7P to d30P (R2 = 0.25, slope = 11, P < 0.01). As with COX-2, GM-CSF was expected to follow the pattern of IFN-т secretion.
When examined separately by regression analysis, the up-regulation of GM-CSF in lE through time was confirmed between d7P and d21P (R2 = 0.38, slope = 14, P < 0.005), but a plateau was rather obtained between d21P and d30p (R2 = 0, slope = -0.5, P > 0.5). When d7, d16, and d18 were compared across status (pregnant vs. cyclic) by regression analysis, no effect of status was found (P > 0.5). Stromal expression of GM-CSF (Fig. 5B) was not statistically altered through time either during the cycle or the pregnancy (regression analysis, P > 0.5). When d7, d16, and d18 were compared across status (pregnant vs. cyclic) by regression analysis, again no effect of status was found (P > 0.1).