Ventolin

Ventolin Inhalers

- Differential Regulation of Colony Stimulating Factor: RESULTS(2)

RESULTS(2)

In E2 + MPA-treated cultures, CSF1 output was increased from the basal level of 0.2 6 0.05 ng/ml/mg protein to 0.7 6 0.14 (P < 0.05) and 0.79 6 0.13 (P < 0.05) ng/ml/ mg protein by TNF and IL1B, respectively. In contrast to the marked elevation of CSF1 elicited by these cytokines, the addition of MPA plus E2 did not significantly alter the levels of CSF1 compared to cultures treated with E2 alone, and did not alter the responses to the cytokines.

In contrast to the effects of TNF (1 ng/ml) and IL1B on CSF1 expression, neither cytokine influenced the MIF levels in decidual cell-conditioned medium (Fig. 3). Specifically, the levels of MIF in TNF (1 ng/ml)- and IL1B (1 ng/ml)-treated cells were 15.8 6 4.4 ng/ml/mg cell protein and 14.6 6 3.45 ng/ml/mg cell protein, respectively, which were not significantly different than the levels in control cultures incubated with E2 alone (16.4 6 3.2 ng/ml/mg cell protein). Similarly, in E2 + MPA-treated cultures, the basal level of MIF was 18.4 6 4.4 ng/ml/mg protein, and this was not changed by treatment with either TNF (12.58 6 2.0 ng/ml/mg protein) or IL1B (15.5 6 2.4 ng/ml/mg protein).

Fig3Differential Regulation of Colony-4
FIG. 3. Effects of E2 MPA, TNF, and IL1B on MIF production by term decidual cell monolayers. Confluent, leukocyte-free decidual cells were incubated for 7 days with 10~8 M E2 or with E2 + 10~7 M MPA, and then switched to DM with the corresponding steroid(s) with or without 1 ng/ml of TNF or IL1B for 24 h. The MIF levels in conditioned DM were measured by ELISA and normalized to the level of cell protein (n = 8, mean ± SEM).

Fig4Differential Regulation of Colony-3
FIG. 4. Effects of E2, MPA, TNF, and IL1B on CSF1 production by first trimester decidual cell monolayers. Confluent, leukocyte-free decidual cells were incubated for 7 days with 10~8 M E2 or with E2 + 10~7 M MPA, and then switched to DM with the corresponding steroid(s) with or without 1 ng/ml of TNF or IL1B for 24 h. The CSF1 levels in conditioned DM were measured by ELISA and normalized to the level of cell protein (n = 6, mean ± SEM). *, versus E2 (P < 0.05);**, versus E2 + MPA (P < 0.05).

Fig5Differential Regulation of Colony-5
FIG. 5. Effects of E2, MPA, TNF, and IL1B on MIF production by first trimester decidual cell monolayers. Confluent, leukocyte-free decidual cells were incubated for 7 days with 10~8 M E2 or with E2 + 10~7 M MPA, and then switched to DM with the corresponding steroid(s) with or without 1 ng/ml of TNF or IL1B for 24 h. The MIF levels in conditioned DM were measured by ELISA and normalized to the level of cell protein (n = 3, mean ± SEM).

July 9, 2014 Macrophage
Tags: cytokines deciduas immunology pregnancy